Abstract: Addressing the requirements for ship’s autonomous navigation and high-precision path following control, this paper proposes a fuzzy control method with partitioned integral regions based on the Integral Line-of-Sight（ILOS） guidance law for path following control of autonomous navigation ships. Based on the established ship motion model, an extended Kalman filter is employed to estimate ship states such as speed and heading using GNSS measurement data. Fuzzy controllers are designed to dynamically adjust the look-ahead distance parameter according to different navigation conditions, thereby improving tracking performance. The 700 Twenty-foot Equivalent Unit（TEU） battery-powered container ship built by COSCO Shipping Group is taken as the plant for path following experiments on a simulation platform.Comparative analyses with results from different algorithms show that the proposed control algorithm achieves superior performance and smoother rudder angle manipulation. This method can provide reference for path following control of autonomous navigation ships.